Ukubuka konke kwe-Lactoperoxidase

I-Lactoperoxidase (LPO), etholakala emithanjeni yokuthambisa kanye ne-mammary, kuyinto ebalulekile yempendulo yokuzivikela komzimba ebalulekile ekugcineni impilo yomlomo. Indima ebaluleke kakhulu ye-lactoperoxidase ukuthola i-oxidize thiocyanate ions (i-SCN−) etholakala kumathe ebusweni be-hydrogen peroxide okuphumela kwimikhiqizo ekhombisa umsebenzi we-antimicrobial. I-LPO etholakala obisini lwe-bovine isetshenzisiwe embonini yezokwelapha, ukudla, nezimonyo ngenxa yokusebenza kwayo nokufana nokwakheka kwe-enzyme yomuntu.

Imikhiqizo yesimanje yokuhlanzeka ngomlomo iyachuma ngohlelo lwe-lactoperoxidase ukuze inikeze enye indlela engcono kakhulu kumazinyo ajwayelekile we-fluoride. Ngenxa yokusetshenziswa okubanzi kwe lactoperoxidase isengezo, isidingo sayo sikhule kakhulu ngokuhamba kweminyaka, futhi sisakhula.
I-Lactoperoxidas-01

Yini iLactoperoxidase?

I-Lactoperoxidase imane nje i-enzyme eyenziwe nge-peroxidase ekhiqizwa kusukela emthanjeni wezinzwa, i-mammary, ne-salivary gland, esebenza njenge-ejenti yemvelo elwa namagciwane. Ezintwini ezingabantu, i-encyme ye-lactoperoxidase ifakiwe uhlobo lwe-LPO. Le enzyme ivame ukutholakala emadabukeni, kufaka phakathi abantu, amagundane, ama-bovine, ikamela, izinyathi, inkomo, imbuzi, u-Ilama nezimvu.

Umsebenzi we-Lactoperoxidase:

LPO I-ejenti esebenza ngempumelelo kakhulu yokulwa nezinkinga. Ukusetshenziswa kwe-lactoperoxidase, ngakho-ke, kususelwa kule nqubo. Isicelo se-Lactoperoxidase ngaleyo ndlela sitholakala ikakhulu ngokulondolozwa kokudla, izixazululo ze-ophthalmic, nezinjongo zokuhlobisa. Futhi, i-lactoperoxidase powder isetshenzisiwe ekwelapheni amanxeba nokwelashwa kwamazinyo. Ngaphezu kwalokho, i-LPO iyi-ejenti ephumelelayo yokulwa ne-viral ne-anti-tumor. Kuxoxwe ngezansi kusetshenziswa ngaphezulu kwe-lactoperoxidase:

i. Umdlavuza wesibeletho

Umdlavuza we-Lactoperoxidase ikhono lokuphatha lihambisana namandla alo oxidize estradiol. Le oxidation iholela ekucindezelekeni kwama-oxidative kumaseli womdlavuza webele. Umsebenzi we-Lactoperoxidase lapha ukudala uchungechunge lokuphendula okuholela ekusetshenzisweni kwe-oksijini kanye nokuqongelela kwe-hydrogen peroxide ye-intracellular. Ngenxa yalokhu kusabela, i-LPO ibulala ngempumelelo amaseli e-tumor ku-vitro. Futhi, ama-macrophages adalulwe kwi-LPO enza kusebenze amasosha omdlavuza, awabulale.

ii. Umphumela olwa namagciwane

I-enzyme ye-LPO isebenza njengezakhi zemvelo zohlelo lokuvikela izivikela olungavikeleli ezifweni futhi ifaka i-oxidation ye-thiocyanate ion kwi-hypothiocyanate ye-antibacterial. I-LPO ingavimbela ukukhula kwezinhlobonhlobo zama-microorganisms ngokusabela kwe-enzymatic okubandakanya ama-ion e-thiocyanate ne-hydrogen peroxide njenge-cofactors. Umsebenzi we-antimicrobial we-LPO ugxilwe ekwakhiweni kwama-ion e-hypothiocyanite ngokusebenzisa ukwenziwa kwama-enzyme. Ama-ion e-Hypothiocyanite ayakwazi ukusabela ngezicubu zamagciwane. Zibuye zidale ukuphazamiseka ekusebenzeni kwama-enzymes athile we-metabolic. I-Lactoperoxidase ibulala amagciwane ama-Gram-negative futhi ivimbele ukukhula nokukhula kwamagciwane anempilo eyi-Gram.

Iii. I-Lactoperoxidase kwezimonyo

Inhlanganisela ye-lactoperoxidase powder, ushukela, i-thiocyanate, iodide,

ne-glucose oxidase, futhi baziwa ukuthi bayasebenza ngokulondolozwa kwezimonyo.I-Lactoperoxidas-02

iv. I-Lactoperoxidase ebisini Ukulondolozwa

Amandla we-lactoperoxidase ekugcinweni kwekhwalithi emsulwa yobisi olungavuthiwe okwesikhathi esithile selisunguliwe emikhakheni eminingana kanye nezifundo zokuhlola ezenziwa ezifundeni ezahlukahlukene zezwe. I-Lactoperoxidase i-preservative ingasetjenziselwa ukugcina ubisi olungavuthiwe olutholakala ezinhlotsheni ezahlukene. Ukuthi indlela isebenza kahle kangakanani kuncike ezicini eziningi. Lezi zinto zifaka ukushisa kobisi ngesikhathi sokwelashwa, uhlobo lokungcola kwe-microbiological, kanye nenani lobisi.

I-Lactoperoxidase inomphumela we-bacteriostatic ebisini lobisi oluncane. Idatha yokucwaninga nolwazi olwenziwe kusuka kwento yangempela yangempela kukhombisa ukuthi i-lactoperoxidase ingasetshenziswa ngaphezu kwemikhawulo yokushisa ye (15-30 degrees Celsius) okukhulunywa ngayo kuzinkombandlela ze-codex zango-1991. Ekupheleni okungenani kwesilinganiso sokushisa, izifundo ezahlukahlukene zibonisa ukuthi kusebenze i-lactoperoxidase kungabambezela ukukhula kwama-bacterium obisi lwe-psychrotrophic futhi ngaleyo ndlela kubambezele inkcitho yobisi izinsuku eziningi kuqhathaniswa nesiqandisi kuphela. Kubalulekile ukuqaphela ukuthi inhloso yokusebenzisa i-lactoperoxidase akuyona ukwenza ubisi luphephe ukuze lusetshenziswe kepha ukulondolozela ikhwalithi yayo yangempela.

Ukuzijwayeza ukuhlanzeka okuhle ekukhiqizeni ubisi kubalulekile ekusebenzeni kwe-lactoperoxidase nekhwalithi yobisi lwe-microbiological. Ukuphepha nokwanda kobisi kungatholakala kuphela ngokuhlanganiswa kokulashwa okushisa kobisi kanye nemikhuba emihle yokuhlanzeka ngaphandle kokusebenzisa i-lactoperoxidase.

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v. Ezinye imisebenzi

Ucwaningo luye lwabonisa ukuthi ngaphezu kokuba nemiphumela yokuvimbela i-antiviral, i-Lactoperoxidase futhi ingavikela amaseli ezilwane emonakalweni ahlukahlukene kanye ne-peroxidation, futhi futhi yingxenye ebalulekile yohlelo lokuvikela ngokumelana nama-microorganisms angama-pathogenic ohlelweni lokugaya izingane ezisanda kuzalwa.

Uhlelo lweLactoperoxidase

Yini Uhlelo Lactoperoxidase?

I-Lactoperoxidase system (i-LPS) yakhiwa ngezinto ezintathu, ezibandakanya i-lactoperoxidase, i-hydrogen peroxide, ne-thiocyanate (SCN¯). Uhlelo lwe-Lactoperoxidase lunemisebenzi yokulwa namagciwane kuphela lapho lezi zinto ezintathu zisebenza ngokubambisana. Ekusetshenzisweni kwangempela kwempilo, uma ukuqoqwa kwento ethile ohlelweni kunganele, kufanele kufakwe ukuze kuqinisekiswe umphumela wokulwa namagciwane, owaziwa ngokuthi yi-LPS activation. Phakathi kwabo, i-lactoperoxidase okuhlushwa akufanele ibe ngaphansi kuka-0.02 U / mL.

Ukuxakaniseka kwemvelo kwe-lactoperoxidase kubisi lwe-bovine yi-1.4 U / mL, engahlangabezana nale mfuneko. I-SCN¯ itholakala kabanzi kuma-secretion nezicubu zezilwane. Ebisini, iqoqo le-thiocyanate lincane njenge-3-5 μg / mL. Lokhu kuyisici esinqunyelwe umsebenzi we-Lactoperoxidase system. Kuphakanyisiwe ukuthi i-thiocyanate edingekayo ukwenza uhlelo lwe-lactoperoxidase luzungeze i-15 μg / mL noma ngaphezulu. Kungakho kudingeka sengeze le thiocyanate exo native ukusebenzisa uhlelo lwe-lactoperoxidase. Okuqukethwe kwe-hydrogen peroxide obisini, okuye kwafakelwa ngaphandle, kungama-1-2 μg / Ml kuphela, futhi ukusebenza kwe-LPS kudinga 8-10 μg / mL ye-hydrogen peroxide. Kungakho i-hydrogen peroxide kufanele inikezwe ngaphandle.

Uhlelo lwe-lactoperoxidase lubamba iqhaza elibalulekile emasosheni omzimba angaphakathi, lungabulala amagciwane kubisi kanye nasekuncinweni kwe-mucosal futhi lungaba nezicelo zokwelapha.

Emikhiqizweni yokudla neyokunakekelwa kwezempilo, ukwengeza noma ukwandisa uhlelo lwe-lactoperoxidase kwesinye isikhathi kusetshenziselwa ukulawula amagciwane.

Isebenza kanjani?

I-LPS iqukethe ukukhiqizwa kwenhlanganisela ye-anti-bacterial evela kwi-SCN¯catalysed yi-LPO phambi kwe-hydrogen peroxide. I-lactoperoxidase eshiwo umsebenzi we-antimicrobial ngokwemvelo itholakala ezintweni eziningana uketshezi lomzimba njengejusi yesisu, izinyembezi kanye namathe. Izici ezimbili ezibalulekile zohlelo lwe-antimicrobial, okuyi-hydrogen peroxide ne-thiocyanate, zikhona ebisini ngokugxila okuhlukile, kuya ngohlobo lwesilwane nokuphakelwa okunikezwe.

Kubisi olusha, umsebenzi we-antimicrobial awunamandla futhi uhlala amahora angama-2 kuphela njengoba ubisi luqukethe kuphela amazinga we-hydrogen peroxide ne-thiocyanate ion. I-Thiocyanate iyanezelwa ekhonjwe ku-2 elektrron reaction reaction hypothiocyanite

I-Thiocyanate isebenza njenge-cofactor yohlelo lwe-Lactoperoxidase. Njengomphumela, inani lama-sulfhydryls oxidized esekonke azimele ion we-thiocyanate inqobo nje

  1. I-Thiol moeches iyatholakala
  2. I-Thiocyanate ayiphelelwa amandla
  • I-hydrogen peroxide eyanele ikhona
  1. I-Thiocyanate okwamanje ayifakwa ku-amino acid enephunga

Ngenxa yalokho, i-thiocyanate iphinda ivuselele umthelela wokulwa namagciwane wesistimu ye-lactoperoxidase kubisi olusha. Lokhu kwandisa impilo yeshalofu yobisi olusha ngaphansi kwezimo ezishisayo amahora ayisikhombisa kuya kwayisishiyagalombili.

I-Lactoperoxidase Isicelo / Ukusetshenziswa

i. Isenzo Esilwa Namagciwane

Umsebenzi we-anti-microbial wohlelo lwe-lactoperoxidase ubonakala esenzweni se-bactericidal kanye ne-bacteriostatic sezinye izidalwa ezincane ezitholakala obisini olungavuthiwe. Umshini wayo we-bactericidal usebenza ukuthi iqembu le-thiol elitholakala kulolwelwesi lwamangqamuzana amancane anobuthi liyakhishwa. Lokhu kuphumela ekubhujisweni kwesakhiwo se-plasma membrane okuholela ekuvuthweni kwama-polypeptides, ion we-potassium, kanye nama-amino acid. Ukuthathwa kwama-purine kanye nama-pyrimidines, ushukela, ne-amino acid ngamangqamuzana kuvinjelwe. I-Synthesis ye-DNA, i-RNA, namaprotheni nayo ivinjiwe.

Amagciwane ahlukahlukene akhombisa amazinga ahlukene ezwela ohlelweni lwe-lactoperoxidase. Amagciwane angenayo i-Gram-negative, anjenge-Salmonella, Pseudomonas, ne-Escherichia coli, akhinyelwa futhi abulawa. Ama-bacterium e-Lactic acid ne-Streptococcus avinjelwe kuphela. Ukubhujiswa kwalawa mabhaktheriya ngohlelo lwe-lactoperoxidase kubangela ukuvuza kwezakhi ezithile zomzimba, kuvimbele amagciwane ekutholeni izakhi zomzimba, futhi lokhu kuholela ekwehleni noma ekufeni kwamagciwane.

ii. Ukwelashwa kwe-paradentosis, i-gingivitis, nokubulala amaseli wamathumbu

I-LPS kukholakala ukuthi uyasebenza ekwelapheni i-gingivitis ne-paradentosis. I-LPO isetshenzisiwe ekushweni komlomo ukwehlisa amagciwane omlomo futhi, ngenxa yalokho, i-acid ekhiqizwa yileli gciwane. Ama-antibody conjugates wohlelo lwe-lactoperoxidase ne-glucose oxidase akhombisile ukuthi asebenza ngempumelelo ekubhubhiseni futhi ngenxa yalokho abulala amaseli we-tumor in vitro. Futhi, ama-macrophages adalulwe ohlelweni lwe-lactoperoxidase asetshenziselwa ukubulala nokubulala amaseli womdlavuza.

Iii. Ukunakekelwa komlomo

Izifundo ezahlukahlukene zokwelapha ezichaza ukusebenza kwe-LPS ekuhlanzeni izinyo ziye zabhalwa phansi. Ngemuva kokukhombisa ngokungaqondile, usebenzisa imingcele yokuhlola izimo zamakhokho, leyo liceoperoxidase yamazinyo aqukethe i-amyloglucosidase (γ-amylase) inomphumela omuhle ekunakekelweni ngomlomo. Ama-enzymes anjenge-glucose oxidase, i-lysozyme, ne-lactoperoxidase adluliswa ngqo kusuka kwizinyo lokuhlanza kuya ku-pellicle.

Ukuba yizingxenye ze-pellicle, la ma-enzyme asebenza kakhulu ngokunamandla. Futhi, i-LPS inomphumela onenzuzo wokuvimba ngokumelene namakhanda okuqala ebuntwaneni ngokwehlisa inani lamakoloni akhiwa yi-microflora ye-cariogenic njengoba ikhulisa ukuqoqwa kwe-thiocyanate.

Ngeziguli ze-xerostomia, amazinyo we-lactoperoxidase iphakeme ngokwedlulele uma iqhathaniswa namazinyo we-fluoride uma kukhulunywa ngokwakhiwa kwama-plaque. Ukusetshenziswa kwe-LPS akuvinjelwe ku-periodontitis kanye namakhokho. Inhlanganisela ye-lactoperoxidase ne-lysozyme zingasetshenziswa ekwelashweni kwe-syndrome yomlomo ovuthayo.

Lapho i-LPS ihlanganiswa ne-lactoferrin, le nhlanganisela ilwa ne-halitosis. Lapho i-LPS ihlanganiswe ne-lysozyme ne-lactoferrin, i-LPS isiza ekwenzeni ngcono izimpawu ze-xerostomia. Futhi, ama-gels anohlelo lwe-lactoperoxidase asiza ukuthuthukisa izimpawu zomdlavuza womlomo lapho ukukhiqizwa kwamathe kungavinjelwe ngenxa yokufakelwa.

I-Lactoperoxidas-04

iv. Ngcono amasosha omzimba

Umsebenzi we-lactoperoxidase antimicrobial udlala umsebenzi obalulekile kumasosha omzimba. I-Hypothiocyanite iyisakhi esisebenzayo ekhiqizwa umsebenzi we-lactoperoxidase ku-thiocyanate. I-hydrogen peroxide ikhishwa ngamaprotheni e-Duox2 (i-oxidase 2). Ukufihlwa kwe-Thiocyanate ezigulini ezine-cystic fibrosis kwehliswa. Lokhu kuphumela ekunciphiseni ukukhiqizwa kwe-antimicrobial hypothiocyanite. Lokhu kufaka engcupheni enkulu yokutheleleka nge-airway.

I-LPS ivimbela i-helicobacter pylori kahle. Kepha kuwo wonke umuntu, i-LPS ikhombisa ukungabi namandla kwamagciwane. I-LPS ayihlaseli iDNA futhi ayisiyo mutagenic. Kepha, ngaphansi kwezimo ezithile, i-LPS ingadala ukucindezela okuncane kwe-oxidative. Kufakazelwe ukuthi i-LPO ebukhoneni be-thiocyanate ingadala imiphumela ye-cytotoxic kanye ne-bactericidal ye-hydrogen peroxide ngaphansi kwezimo ezithile, kufaka phakathi lapho i-H2O2 ikhona ekuxubeni kokuphendula ngokweqile kwe-thiocyanate.

Ngaphezu kwalokho, ngenxa yezakhiwo zayo ezinamandla nezisebenzayo ze-antibacterial kanye nokumelana nokushisa okuphezulu, isetshenziswa njenge-ejenti elwa namagciwane ekunciphiseni imiphakathi yama-bacteria emikhiqizweni yobisi noma yobisi futhi njengesikhombi sobisi lwe-ultra-pasteurization. Ngokusebenzisa uhlelo lwe-lactoperoxidase, impilo yeshelufu yobisi oluhlaza okuqandisiwe nayo ingandiswa.

Futhi, i-hypothiocyanate ekhiqizwa yi-lactoperoxidase ingasetshenziselwa ukunqabela igciwane le-herpes simplex kanye negciwane lesifo sokugonywa komuntu.

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Ingabe kuphephile impilo yabantu nezilwane?

Iminyaka eyishumi nanhlanu yezifundo zensimu emazweni asathuthuka nasethuthukile yenziwa futhi yahlolwa yi-FAO / WHO JECFA (Komiti Yezabahlanganyeli Zabahlanganyeli Kwezokudla). Ngemuva kokuba lezi zinhlolokhono ezijulile futhi ezinkulu zaphothulwa, ukusetshenziswa kohlelo lwe-lactoperoxidase ekugcinweni kobisi kwavunywa yi-FAO / WHO JECFA (Ikomidi Lezazi ngokudla okungeziwe). Ochwepheshe baphinde bathi le ndlela iphephile empilweni yabantu nasezilwaneni.

I-LPS iyisimo esingokwemvelo sejusi esiswini namathe kubantu, ngakho-ke, iphephile uma isetshenziselwa ukunamathela Kuzinkombandlela zeKhodi yeCodex Alimentarius Commission. Le ndlela ayizithinti izilwane ezikhanyayo noma yini. Lokhu kungenxa yokuthi ukwelashwa kwenziwa kuphela emva kokuba ubisi selukhishwe emhlangeni.

Isiphetho

Kuyabonakala engxoxweni yethu ukuthi uhlelo lwe-lactoperoxidase kanye ne-lactoperoxidase lusebenza kakhulu futhi luwusizo kakhulu ezinhlobonhlobo zezicelo. Uma ufuna ukwenza ophelele lactoperoxidase buy ocwaningweni lwakho noma ekuthuthukiseni izidakamizwa, ungabe usabheka okunye. Sikwazi ukucubungula ama-lactoperoxidase ama-bulk amaningi ngesikhathi esifushane kakhulu futhi siwathumele e-US, eYurophu, eCanada nakwezinye izingxenye zomhlaba. Zizwe ukhululekile ukuxhumana nathi ukuthola eminye imininingwane.

Okubhekwayo

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  2. UTenovuo JO (1985). "Uhlelo lwe-peroxidase ekusithekeni kwabantu." KuTenovuo JO, uPrut KM (ed.). Uhlelo lwe-Lactoperoxidase: chemistry nokubaluleka kwezinto eziphilayo. ENew York: UDekker. k. 272.
  3. UThomas EL, u-Bozeman PM, Funda i-DB: I-Lactoperoxidase: isakhiwo nezakhiwo ezinamandla. Ama-Peroxidases ku-Chemistry ne-Biology. Kuhlelwe ngu: Everse J, Everse KE, Grisham MB. 1991, iBoca Raton, FL. CRC Press, 123-142.
  4. Wijkstrom-Frei C, El-Chemaly S, Ali-Rachedi R, Gerson C, Cobas MA, Forteza R, Salathe M, Conner GE (Agasti 2003). "I-Lactoperoxidase kanye nomoya wokuzivikela womuntu oqhuba umoya". Am. J. Respir. Iseli Mol. Biol. 29 (2): 206–12.
  5. UMikola H, uWaris M, uTenovuo J: Ukuvinjwa kohlobo lwegciwane le-herpes simplex 1, igciwane lokuphefumula lokuphefumula, nohlobo lwe-echovirus 11 ngohlobo lwe-hypoxocaseite eyenziwe yi-peroxidase. I-Antiviral Res. 1995, 26 (2): 161-171.
  6. IHaukioja A, Ihalin R, Loimaranta V, Lenander M, Tenovuo J (Septhemba 2004). "Ukuzwela kwe-Helicobacter pylori kumshini wokuzivikela ongaphakathi, uhlelo lwe-lactoperoxidase, ku-buffer nakuwo wonke amathe abantu". I-Journal of Medical Microbiology. 53 (Pt 9): 855-60.

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